A Semi-Automated, Non-Radioactive Assay for the Detection of Antibody-Based Complement-Dependent Cytotoxicity
نویسنده
چکیده
Pharmaceutical companies are increasingly focused on biologic therapeutics rather than biochemical, chemical and radiological compounds for many cancer treatments. Biologic therapeutics only target and destroy specific cancer cells, thus preventing damage to surrounding, healthy cells and reducing unwanted cytotoxic events. The most prominent and successful example is Rituximab (Rituxan®; Roche/Genentech), a chimeric monoclonal antibody (mAb) therapy that is used for certain cancers and autoimmune disorders.
منابع مشابه
Real Time Assays for Quantifying Cytotoxicity with Single Cell Resolution
A new live cell-based assay platform has been developed for the determination of complement dependent cytotoxicity (CDC), antibody dependent cellular cytotoxicity (ADCC), and overall cytotoxicity in human whole blood. In these assays, the targeted tumor cell populations are first labeled with fluorescent Cell Tracker dyes and immobilized using a DNA-based adhesion technique. This allows the fac...
متن کاملDetection of HLA Antibodies in Organ Transplant Recipients – Triumphs and Challenges of the Solid Phase Bead Assay
This review outlines the development of human leukocyte antigen (HLA) antibody detection assays and their use in organ transplantation in both antibody screening and crossmatching. The development of sensitive solid phase assays such as the enzyme-linked immunosorbent assay technique, and in particular the bead-based technology has revolutionized this field over the last 10-15 years. This revol...
متن کاملMethodologies for anti-HLA antibody screening in patients awaiting kidney transplant: a comparative study.
OBJECTIVES The relevance of anti-HLA antibodies in patients awaiting kidney transplants is well recognized. During the past 40 years, kidney transplant candidates have been tested for these antibodies, and the choice of the detection assay has become essential. Recently, the pioneer method, the complement-dependent cytotoxicity, has been integrated but has not been replaced by more-sensitive so...
متن کاملDevelopment of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus
Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...
متن کاملThe Toxicity of Thorium Oxide Nanoparticles Conjugated with Folic acid and Antibody on the Mononuclear Cells from Patients with Chronic lymphoid Leukemia and Normal Persons
Background and Aims: The aim of this study was to synthesize antibody-conjugated radioactive(thorium oxide) nanoparticles (ACRNPs) and folic acid-conjugated radioactive nanoparticles (FACRNPs). Materials and Methods: After synthesis of nanoparticles, blood samples from CLL patients and normal subjects were obtained, and their mononuclear cells were isolated by Ficoll method. To evaluate cyto...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2012